SPIN-LABELED NUCLEIC ACIDS
نویسندگان
چکیده
منابع مشابه
Pulsed Electron Double Resonance in Structural Studies of Spin-Labeled Nucleic Acids
This review deals with the application of the pulsed electron double resonance (PELDOR) method to studies of spin-labeled DNA and RNA with complicated spatial structures, such as tetramers, aptamers, riboswitches, and three- and four-way junctions. The use of this method for studying DNA damage sites is also described.
متن کاملAnalysis of microliter volumes of dye-labeled nucleic acids.
We describe methodology useful for determining the fluorescent activity of small volumes (e.g., 1 microL) of dye-labeled cDNAs using microabsorbance spectroscopy. A direct relationship was found between the average fluorescent hybridization signal from an array and the fluorescent activity of the cDNA used in the hybridization. The microabsorbance method makes possible the screening of small-vo...
متن کاملStudies on spin labeled ribonucleic acids encapsulated by viral proteins.
Spin labeled poly rA (sl-poly rA) was encapsulated by the coat proteins of two plant viruses having different morphologies: TMV, a rigid rod and CCMV, an icosahedral sphere. Electron microscopy showed that the resultant particles were morphologically similar to the parent virus from which the coat protein was obtained. Encapsulation produced progressive immobilization of the spin label. The mot...
متن کاملNoncovalent and site-directed spin labeling of nucleic acids.
Electron paramagnetic resonance (EPR) spectroscopy is widely used to study free radicals or paramagnetic centers associated with biopolymers. With the advent of pulsed EPR methods, which allow accurate distance measurements between 20 and 80 , structures of biopolymers have increasingly been interrogated by this technique. Some of the advantages of EPR spectroscopy over other structural techniq...
متن کاملSite-Directed Spin Labeling for EPR Studies of Nucleic Acids
Electron paramagnetic resonance (EPR) spectroscopy has emerged as a valuable technique to study the structure and dynamics of nucleic acids and their complexes with other biomolecules. EPR studies require incorporation of stable free radicals (spin labels), usually aminoxyl radicals (nitroxides), at specific sites in the nucleic acids using site-directed spin labeling (SDSL). In addition to the...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1967
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.58.3.884